ozonated oil

What is Ozonated Oil?

Ozonated Oil products (based on olive oil, sunflower oil or others) provide a variety of scientifically and medically proven health benefits (see references below). They are used in cosmetics, skin care, beauty care, medical / pharmaceutical, food and animal products.

Some of the main benefits and typical uses of Ozonated Oils include:

  • Regenerates and repairs the skin helping the healing process
  • Protects, moisturises and nourishes the skin
  • Cleans and sterilizes the epidermis
  • Improves blood circulation
  • Controls and prevents the spread of germs in some skin conditions (seborrhoea, acne, ringworm, skin lesions).
  • Reduces swelling and inflammation
  • Improves cellular function and cellular memory
  • Helps repair damaged skin conditions such as redness, bedsores, sunburn, wrinkles,
  • Helps avoid the use of more aggressive medical treatments such as corticosteroid creams
  • Aids in the healing processes reduces scarring, soothes sore muscles
  • Reduces incidence of degenerative skin conditions
  • Remedy for sunburn, wrinkles, insect bites and stings
  • Infections of the sebaceous glands (sweat)
  • Other uses: haemorrhoids, cuts and burns, skin yeast, tinea versicolor, dermatitis

How are Ozonated Oils made?

Ozonated Oils are made from lipid oxidation, a product of the reaction of ozone with fatty acids and other substrates contained in vegetable oils. These compounds have germicidal, immunostimulant and tissue repair activity, which is why they are being applied today as a new strategy for the treatment of infections and different pathologies.1-4

Ozone is a strong oxidiser which can kill microorganisms and viruses effectively. It has been used for treatment of water and wastewater since the early 1900s. Ozone has a very short life time and at room temperature. It returns to its oxygen state in less than a minute, limiting its use. This is where vegetable oils come in. When mixed with ozone it modifies the chemical structure of vegetable oils and allows the ozone to be stored in a stable and active manner for several years. 1-4

Through these ozone / vegetable oil chemical reactions, pharmacological active ingredients are produced with high added value without changing the rest of the molecule. The compounds that are formed when ozonising the oil are: ozonides, peroxides and aldehydes; all three are substances with proven germicidal properties.1-6 The physical properties of the oil allow it to penetrate places where other aqueous antibiotics do not reach, such as alveolitis in the root canals of the teeth.1-6

Ozonated oil therapy
“The art of medicine consists in amusing the patient while nature cures the disease.” –  Voltaire


Since the late 1800s, the use of sunflower, coconut and cod liver oils has been described in patients with different conditions, mainly tuberculosis. The oils were prepared by enriching them with oxygen and exposing them to UV radiation from the sun.7

The applications for ozonated vegetable oil have spread rapidly. They have been used for the topical treatment of various diseases such as: skin infections, nail fungus, diabetic foot, parasitosis, papillomatosis, traumatic lesions, infected wounds, ulcers, eschar, herpetic lesions, fungal infections, insect bites, dermatitis, skin in an inflammatory phase due to atopic dermatitis, interdigital itching, psoriasis, vaginal infections and disorders, burns, and infectious stomatological conditions, among many others.7-25

Additionally, Ozonated Oils have other properties that are quite important: stability, long shelf life, tolerance, low surface tension, it is soluble in fat and does not dry out, thus preventing the formation of scabs and cracks.7,22

PathologiesSpecific typesProduct
PeriodontalAcute gingivitis Acute necrotizing ulcer gingivitis PeriodontitisOzonated sunflower oil, topical application
StomatologicalAphthous stomatitis Infected root canals AlveolitisOzonated sunflower oil, topical application
Venous insufficiency of the lower limbsVaricose ulcersLocally applied ozonated oil emulsion
Stomach / digestive systemGastric ulcers Intestinal infections Giardiasis Helicobacter pylori Hemorrhoids Acute tonsillitisCapsules, oral Capsules, oral Capsules, oral Capsules, oral Topical application Topical application
DermatologicalAcne Athlete´s foot (tinea pedis) Impetigo Onychomycosis Fistulas Surgical wounds Burns Pressure sores Folliculitis and bedsores Diabetic footTopical application
OphthalmologicalConjunctivitis Corneal ulcers Keratitis Blepharitis Viral keratitisTopical application
GynecologicalHuman papillomavirus (HPV) Vaginal level and cervix (94% efficacy) Genital herpes Vulvovaginitis due to Candida AlbicansApplication topically and ovules
Fig.1 Summary of Ozonated Oil applications for different pathologies

Why use concentrated ozone in an oil?

The answer is simple: many of the drugs that previously were efficient, have stopped giving the expected results. In some cases the doses have to be increased significantly and in others, the side effects are very harmful.

Are all vegetable oils useful in the preparation of Ozonated Oil?

Before answering this question, we could ask the following question: where do vegetable oils come from? They are a reserve food product and are necessary for plant cells to start the germination process of seeds inside some fruits.

ozonated oil therapy

Vegetable oils are mainly made up of triglycerides and, depending on their origin, their composition of saturated and unsaturated fatty acids can vary (Fig.2).26 All vegetable oils can, in principle, be ozonized. However, the reaction of ozone with vegetable oils occurs through the double bonds of unsaturated fatty acids (Fig.3). Hence the greater the number of double bonds available in the molecule, the greater the number of possible reactions with ozone and also the higher activity of the final product.

Vegetable OilSaturated (%)Monounsaturated (%)Polyunsaturated (%)
Fig.2 Composition of some vegetable oils.
Classification of fatty acids
Fig. 3 Classification of fatty acids commonly found in vegetable oils.

One of the oils that has given the best results, in terms of its antimicrobial activity, is sunflower seed oil.3,19 This is attributed, particularly, to the amount of polyunsaturated fats it contains.

Oils hold varying amounts of ozone depending on the oil, which can be measured. The Potential Ozonide Index (POI) is determined by measuring the Omega 3, 6, and 9 fatty acids in the oil. Oils with higher 9s hold more ozone. Each oil has a different amount of 3, 6, and 9 fatty acids and each fatty acid is weighed differently. Every one has its own unique benefits as well as different level of ozone saturation levels it can obtain. Coconut holds lower amounts of than other oils, making it ideal for those who want to use ozone but prefer lower levels of intensity, for example.

Below is a chart indicating the POI for some common vegetable oils. The Formula used is:

Omega 3 (x3) + Omega 6 (x2) + Omega 9 (x1) = POI

Vegetable OilOmega 3Omega 6Omega 9Strength (POI)
Fig. 4 POI (Potential Ozonide Index) for some common vegetable oils.

What are the benefits of Ozonated Oil?

Some of the benefits of Ozonated Oil include:

  • It is 100% organic.
  • Does not require additives such as stabilizers, preservatives and colorants.
  • In topical application it is activated by the body temperature releasing oxygen to the skin.
  • The release process can take up to twelve hours or more (extended release).
  • It is safe and non-toxic.
  • It is one of the most advanced and proven products on the market. Ozonated Oil has been known since the 1800s. Many current patents have existed for 40 years.
  • It is so stable that it can last indefinitely if kept refrigerated.
  • Has similar or superior effects to traditional antibiotics.
  • A broad antimicrobial spectrum
  • A low rate of adverse events
  • Lower cost compared to conventional treatments

What type of products are marketed using Ozonated Oil as the active ingredient?

Ozonated Oil products that are being marketed worldwide include: extracts, creams, ointments, gels, ovules, soft capsules and suppositories among others.

Ozonated Oils are also used extensively in the cosmetic market as the active ingredient for antibacterial soaps, deodorants (they do not require aluminum hydroxide), toothpastes, bath gels, anti-aging creams, regenerative creams and special creams (for normal, dry, sensitive and oily skin types).

Currently, there are a wide variety of products on the market for specific ailments and conditions that use Ozonated Oil as the main active ingredient. For example, to fight acne, since it acts directly against the propionibacterium acnes bacteria.

The application of Ozonated Oil has spread to many countries. Ozonated Oil is considered by many doctors around the world as an alternative of great value because not only does it replace a large number of medicines but it is also relatively low cost, compared to conventional therapies, making it quite attractive.

ozonated oil


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Evans TJ, “What is ozone therapy?”. Concinnity Living, 5Nov20.

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2.   Bocci V. “Ozone A New Medical Drug”. Holland: Springer; 2005. 295 p.

3.   Sechi LA et al. “Antibacterial activity of ozonized sunflower oil (Oleozon)”. J Appl Microbiol. Feb 2001;90(2):279-84.

4.   Viebahn-Hänsler R. “The use of ozone in medicine”. 5th ed. Deutschland: ODREI; 2007. 1-176 p.

5.   El Hadary AA et al. “Evaluation of the effect of ozonated plant oils on the quality of osseo integration of dental implants under the influence of cyclosporin a: an in vivo study”. Implantol, J Oral. 2011;37(2):247-57.

6.   Goldstein LS. “The treatment of acute herpetic gingivostomatitis with ozonide of olive oi”. J Med, NY State. 1950;50(10):1252-1260.

7.   G. Martínez- Sánchez, L. et al. “Las aplicaciones médicas de los aceites ozonizados, actualización”. Rev Esp de  Ozonoterapia. (2012);  2(1): 121-139.

8.   WO0137829(A1), Gomez-Moraleda MA, Melegari P, Aglio RD. “Composition comprising ozonized oils and/or other ozonized natural and/or synthetic products and their use in pharmaceutical, cosmetic, dietetic or food supplement compositions in human and veterinary medicine”. 2001.

9.   US984722, Twombly AH; “Composition Yielding Ozone”. 1911.

10.  US5270344, Herman S; “Method of treating a systemic disorder using trioxolane and diperoxide compounds” 1993.

11.  US5364879, Herman S; “Medical uses of trioxolane and diperoxide compounds”. 1994.

12.  US2356062, Charles J. “Therapeutic Composition”. 1944.

13.  US3504038, Beal RE. “Ozonization of vegetable oils in an improved aqueous medium”. 1970.

14.  Zamora Z, Gonzalez R, et al. “Ozonized sunflower oil reduces oxidativedamage  induced by indomethacin in rat gastric mucosa”. Res, Inflamm. 2008;57(1):39-43.

15.  Zamora Rodriguez ZB, Gonzalez Alvarez R, et al. “Antioxidant mechanism is involved in the gastro-protective effects of ozonized sunflower oil in ethanol-induced ulcers in rats”. Inflamm, Mediators. 2007;2007:65873.

16.  Valacchi G, Fortino V, Bocci V. “The dual action of ozone on the skin”. Br J Dermatol. 2005;153(6):1096-100.

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18.  Alfonso K, Collazo M, Fernandez M, Ballagas C. “Cost-efficacy Analysis of Topical Ozonized Oil versus Mupirocin Cream in the Treatment of Impetigo”. Pharm, Latin Am J. 2008; 27(4):512.

19.  Menendez S, Falcon L, Maqueira Y. “Therapeutic efficacy of topical: Oleozon in patients suffering from onychomycosis”. Mycoses. 2011; 54(5): 272-277.

20.  Matsumoto A, Sakurai S, N NS. “Therapeutic effects of ozonized olive oil in the treatment of intractable fistula and wound after surgical operation”. In: IOA MTC (ed). 15th Ozone World Congress. London, UK: Speedprint Mac Media Ltd, 2001:77–84

21.  Martinez-Sanchez G, Al-Dalain SM, et al. “Therapeutic efficacy of ozone in patients with diabetic foot”. Eur J Pharmacol. 2005;523(1-3):151-61.

22.  Jordan L, Beaver K, Foy S. “Ozone treatment for radiotherapy skin reactions: is there an evidence base for practice?” Eur. J. Oncol Nurs. 2002;6(4):220-7.

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24.  4° Congreso internacional de Ozonoterapia, Univ. Rey Juan Carlos, Aepromo, Madrid 5-7, Junio 2014, Alcorcon, Madrid: “Nuevos adelantos en terapias de Ozono y productos para Uso Médico, Odontológico  y Veterinario”.

25.  Sifontes, Angela B., “Estudio y Optimización del empleo del ozono como método de tratamiento de muestra en el análisis de aceites vegetales comestibles mediante ETAAS”. Seminario de investigación I, Escuela de Química, Facultad de Ciencias- Universidad Central de Venezuela. Caracas Mayo 2003.

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1 thought on “What is Ozonated Oil?

  • Preventing COVID-19 with Ozone - Wholesale natural products | Nanako August 30, 2021 at 17:12

    […] ozone is a gas and also unstable, it is not easy to apply or administer. The development of Ozonated (or Ozonized / Ozonised) Oil (HOO) solved this problem making ozone highly stable and bioavailable due to its bond with the lipid […]

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